5-I-dU-CE Phosphoramidite

5'-Dimethoxytrityl-5-iodo-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C39H46IN4O8P
M.W.:
856.69
F.W.:
416.07
CAS Number:
178925-48-9
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Mild deprotection: Ammonium Hydroxide for 24 hours at room temperature
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1091-02 0.25 g .25grams 2.92 84 50.4 31.5 22.91 16.8 4.2
10-1091-90 100 µmol .086grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1091-02 0.25 g .25grams 4.36 0.07 80.8 50.5 36.73 5.05
10-1091-90 100 µmol .086grams 1.5 0.07 23.6 14.75 10.73 1.48


Product FAQs

  • What are the extinction coefficients for propynyl-dC and dU? Also Halogenated dC and dU derivatives? Others?
  • What methods are available for crosslinking DNA with DNA, RNA, proteins?