5'-Hexynyl Phosphoramidite

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10-1908
5-Hexyn-1-yl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C15H27N2O2P
M.W.:
298.36
F.W.:
160.11
Catalog Number: Select Pack Size and Format to Reveal

Description

Oligonucleotides prepared using 5’-Hexynyl Phosphoramidite are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1908-02 0.25 g .25grams 8.38 266 159.6 99.75 72.55 53.2 13.3
10-1908-90 100 µmol .03grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1908-02 0.25 g .25grams 12.51 0.07 243.8 152.38 110.82 15.24
10-1908-90 100 µmol .03grams 1.5 0.07 23.6 14.75 10.73 1.48