5-F-dU-CE Phosphoramidite

5'-Dimethoxytrityl-5-fluoro-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C39H46FN4O8P
M.W.:
748.79
F.W.:
308.16
CAS Number:
142246-63-7
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 24 hours

Technical Documents


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1092-02 0.25 g .25grams 3.34 98 58.8 36.75 26.73 19.6 4.9
10-1092-90 100 µmol .075grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1092-02 0.25 g .25grams 4.98 0.07 93.2 58.25 42.36 5.83
10-1092-90 100 µmol .075grams 1.5 0.07 23.6 14.75 10.73 1.48