5'-Cholesteryl-TEG Phosphoramidite

10-O-[1-propyl-3-N-carbamoylcholesteryl]-triethyleneglycol-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C46H82N3O7P
M.W.:
820.13
F.W.:
682.89
CAS Number:
1325147-19-0
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Potential therapeutic oligonucleotides must permeate the cell membrane for optimal activity. The addition of lipophilic groups to an oligonucleotide would be expected to enhance cellular uptake/membrane permeation. The use of Cholesterol-labelled oligos and the consequent improvement in activity has been described. We have designed our Cholesteryl products with triethyleneglycol (TEG) spacers for maximum solubility.

Details

Usage

  • Coupling: 3 minute coupling time.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1976-02 0.25 g .25grams 3.05 88.33 53 33.13 24.09 17.67 4.42
10-1976-90 100 µmol .082grams 1 20 12 7.5 5.45 4 1
10-1976-95 50 µmol .041grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1976-02 0.25 g .25grams 4.55 0.07 84.6 52.88 38.45 5.29
10-1976-90 100 µmol .082grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1976-95 50 µmol .041grams 0.75 0.07 8.6 5.38 3.91 0.54