5'-Bromohexyl Phosphoramidite

6-Bromo-hex-1-yl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C15H30BrN2O2P
M.W.:
381.29
F.W.:
Bromide: 243.04| Azide: 205.15
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Specific to the 5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5’-azido group by displacement of bromide using sodium azide.2

Details

Usage

  • Coupling: 3 minute coupling recommended.
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_10-1946.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1946-02 0.25 g .25grams 6.56 205.33 123.2 77 56 41.07 10.27
10-1946-90 100 µmol .038grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1946-02 0.25 g .25grams 9.79 0.07 189.4 118.38 86.09 11.84
10-1946-90 100 µmol .038grams 1.5 0.07 23.6 14.75 10.73 1.48

References

REFERENCES|(2) J. Lietard, A. Meyer, J.J. Vasseur, and F. Morvan, Tetrahedron Letters, 2007, 48, 8795-8798.