5-Br-dU-CE Phosphoramidite

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10-1090
5'-Dimethoxytrityl-5-bromo-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C39H46BrN4O8P
M.W.:
809.69
F.W.:
369.07
Catalog Number: Select Pack Size and Format to Reveal

Description

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Ammonium Hydroxide: room temperature as required by nucleobases.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1090-02 0.25 g .25grams 3.09 89.67 53.8 33.63 24.45 17.93 4.48
10-1090-90 100 µmol .081grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1090-02 0.25 g .25grams 4.61 0.07 85.8 53.63 39 5.36
10-1090-90 100 µmol .081grams 1.5 0.07 23.6 14.75 10.73 1.48


Product FAQs

  • What are the extinction coefficients for propynyl-dC and dU? Also Halogenated dC and dU derivatives? Others?
  • What methods are available for crosslinking DNA with DNA, RNA, proteins?