5-Br-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-5-bromo-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C46H51BrN5O8P
M.W.:
912.82
F.W.:
368.08
CAS Number:
178925-43-4
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Mild deprotection: Ammonium Hydroxide for |24 hours at room temperature.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1080-02 0.25 g .25grams 2.74 78 46.8 29.25 21.27 15.6 3.9
10-1080-90 100 µmol .091grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1080-02 0.25 g .25grams 4.09 0.07 75.4 47.13 34.27 4.71
10-1080-90 100 µmol .091grams 1.5 0.07 23.6 14.75 10.73 1.48


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