Glen Research’s agreement with ELITechGroup, formerly Epoch Biosciences, allows us to offer several of their proprietary products designed for the synthesis of novel DNA probes. We are pleased to offer products based on ELITechGroup’s Redmond Red®, Yakima Yellow® and AquaPhluor® 593 fluorophores and Eclipse® non-fluorescent quencher. Under our agreement we also supply PPG, a modified nucleoside, and 5’-Aldehyde-Modifier C2 Phosphoramidite. The fluorescent dyes, Yakima Yellow, Redmond Red and AquaPhluor 593, are available as phosphoramidites and supports. Yakima Yellow has an absorbance maximum at 530 nm and emission maximum at 549 nm, Redmond Red’s absorbance and emission maxima are at 579 nm and 595 nm, respectively, and AquaPhluor 593 has an absorbance maximum at 593 nm and emission maximum at 613 nm. The fluorescein alternative, Gig Harbor Green, has been discontinued.
The Eclipse quencher from ELITechGroup solves most of the problems inherent in the synthesis of molecular beacon and FRET probes. The Eclipse molecule is highly stable and can be used safely in all common oligo deprotection schemes. The absorbance maximum for Eclipse Quencher is at 522 nm, compared to 479 nm for dabcyl. In addition, the structure of the Eclipse Quencher is substantially more electron deficient than that of dabcyl and this leads to better quenching over a wider range of dyes, especially those with emission maxima at longer wavelengths (red shifted) such as Redmond Red and Cyanine 5. In addition, with an absorption range from 390 nm to 625 nm, the Eclipse Quencher is capable of effective performance in a wide range of colored FRET probes.
Coupling: A 3 minute coupling time is recommended.
Deprotection: Use AMA and deprotect as required by the nucleobases. With ammonium hydroxide, deprotect at 65 °C/2h or RT/17h for excellent results; 55 °C/17h will yield acceptable results. A minor side reaction leading to a non-fluorescent impurity during deprotection with ammonium hydroxide can occur when using a fast cleaving support, e.g., Glen UnySupport FC. Treatment of the support prior to cleavage/deprotection with a hindered base, such as 10% diethylamine in ACN, for 2-3 minutes will prevent this side reaction.
Freezer storage, -10 to -30°C, dry
This product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of ELITechGroup and is made and sold under license from ELITechGroup. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from ELITechGroup. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). AquaPhluorÂ®, Yakima YellowÂ®, Redmond RedÂ® and EclipseÂ® are registered trademarks of ELITechGroup.