5'-Amino-Modifier C3-TFA

3-(Trifluoroacetylamino)propyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C14H25N3O3PF3
M.W.:
371.34
F.W.:
137.08
CAS Number:
853955-89-2
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research 5'-Modifiers are designed for use in DNA synthesizers to functionalize the 5'-terminus of the target oligonucleotide. The 5'-Amino-Modifiers are available with a variety of chain lengths to fit exactly the desired application. The DMS(O)MT-protected amino group is easier to deprotect compared to the MMT-protected one. The sulfoxy derivative survives conditions of oligonucleotide synthesis and can either be cleaved with standard deblock solution, or left intact for HPLC purification. At the same time, the DMS(O)MT group is fully compatible with cartridge purification. When detritylation on a cartridge is carried out, the DMS(O)MT+, which is more stable than MMT+, does not reattach itself to an amine. We now offer 5'-DMS(O)MT-Amino-Modifier C6 utilizing this new trityl based protecting group. 5'-Amino-Modifier TEG, a hydrophilic triethylene glycol ethylamine derivative, is 12 atoms in length and fully soluble in aqueous media.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1923-02 0.25 g .25grams 6.73 211 126.6 79.13 57.55 42.2 10.55
10-1923-90 100 µmol .037grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1923-02 0.25 g .25grams 10.05 0.07 194.6 121.63 88.45 12.16
10-1923-90 100 µmol .037grams 1.5 0.07 23.6 14.75 10.73 1.48