5'-Amino-dT-CE Phosphoramidite

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10-1932
5'-monomethoxytritylamino-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C39H48N5O6P
M.W.:
713.81
F.W.:
303.21
CAS Number:
194034-71-4
Catalog Number: Select Pack Size and Format to Reveal

Description

5'-Amino-dT terminates an oligonucleotide with a 5'-amino group which may be used for attaching a peptide or a PNA sequence.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Deprotect as required by nucleobases.WARNING: As with other trityl-protected amines, drying down the oligo after cleavage and deprotection without addition of an non-volatile base (for example, TRIS) will lead to loss of the Trityl protecting group. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_MMT_Amino_Modifiers.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1932-02 0.25 g .25grams 3.5 103.33 62 38.75 28.18 20.67 5.17
10-1932-90 100 µmol .071grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1932-02 0.25 g .25grams 5.23 0.07 98.2 61.38 44.64 6.14
10-1932-90 100 µmol .071grams 1.5 0.07 23.6 14.75 10.73 1.48