4-Thio-U-TOM-CE Phosphoramidite

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10-3052
5'-Dimethoxytrityl-4-(2-cyanoethylthio)-Uridine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C52H72N5O9PSSi
M.W.:
1002.29
F.W.:
322.22
Catalog Number: Select Pack Size and Format to Reveal

Description

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Details

Usage

  • Coupling: Synthesize oligoribonucleotide using standard TOM RNA chemistry (6 min. coupling time).
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_4-Thio-U-TOM.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3052-02 0.25 g .25grams 2.49 69.67 41.8 26.13 19 13.93 3.48
10-3052-90 100 µmol .1grams 1 20 12 7.5 5.45 4 1
10-3052-95 50 µmol .05grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3052-02 0.25 g .25grams 3.72 0.07 0 0 14.17 0
10-3052-90 100 µmol .1grams 1.5 0.07 0 0 4.92 0
10-3052-95 50 µmol .05grams 0.75 0.07 0 0 1.79 0