4-Thio-dT-CE Phosphoramidite

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10-1034
5'-Dimethoxytrityl-2'-deoxy-4-(2-cyanoethylthio)-Thymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C43H52N5O7PS
M.W.:
813.95
F.W.:
320.26
CAS Number:
1290537-73-3
Catalog Number: Select Pack Size and Format to Reveal

Description

The C-nucleoside 2'-deoxypseudouridine, in contrast to dU, forms stable C:pseudoU-A triplets. 2-Aminopurine lacks groups critical for hydrogen bonding and is a mildly fluorescent base. Demand for sulfur modified bases continues to expand for investigations of oligonucleotide structure, but primarily for cross-linking purposes. 6-Thio-dG, 4-Thio-dT and 4-thio-dU are very useful modifications for photo cross-linking and photoaffinity labelling experiments. Oligos containing 2-thio-dT are useful in examining protein-DNA interaction by acting as photosensitizing probes. The thiocarbonyl group in 2-thio-dT is especially interesting in that it is available to react with compounds associating with the minor groove of DNA. 2-Amino-A forms a very stable base pair with T containing three hydrogen bonds but the stability of the base pair with 2-thio-T is greatly diminished. Due to steric interactions between the 2-thio group of thymidine and the 2-amino group of 2-amino-A, the base pair contains only a single hydrogen bond. Oligos containing 2-amino-dA and 2-thio-dT exhibit high affinity for natural oligonucleotides but show little affinity for other similar oligos even of a complementary sequence.

Details

Usage

  • Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation.
  • Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 2hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1034-02 0.25 g .25grams 3.07 89 53.4 33.38 24.27 17.8 4.45
10-1034-90 100 µmol .081grams 1 20 12 7.5 5.45 4 1
10-1034-95 50 µmol .041grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1034-02 0.25 g .25grams 4.58 0.07 85.2 53.25 38.73 5.33
10-1034-90 100 µmol .081grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1034-95 50 µmol .041grams 0.75 0.07 8.6 5.38 3.91 0.54


Product FAQs

  • Can 4-thiodU be used for crosslinking and how is it prepared?