3'-dA-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-3'-deoxyAdenosine,2'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C47H52N7O7P
M.W.:
857.95
F.W.:
313.21
CAS Number:
207347-42-0
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Cellular DNA and RNA are made up of ribo- and 2'-deoxyribonucleic acids linked together via 3'-5' phosphodiester linkages and by far comprise the bulk of polynucleic acids found in cells. Much less common are oligonucleotides which have 2'-5' linkages. However, a unique feature of 2'-5' linked oligonucleotides is their ability to bind selectively to complementary RNA. These features suggest a number of interesting uses for 2'-5' linked oligos such as their use as RNA specific probes or in antisense oligos. Recently, oligos have been synthesized using 3'-deoxy-2'-phosphoramidites and 2'-deoxy-3'-phosphoramidites to produce chimeras with 2'-5' linked ends and 3'-5' linked central regions. It was found that 2'-5' phosphorothioate oligos: 1) bind selectively to complementary RNA with the same affinity as phosphodiester oligos; 2) exhibit much less nonspecific binding to cellular proteins; 3) do not activate RNase H. A 3'-deoxynucleoside at the 3'-terminus of an otherwise normal oligonucleotide effectively blocks polymerase extension.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability Similar to dA,C,G,T-CE Phosphoramidites


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1004-02 0.25 g .25grams 2.91 83.67 50.2 31.38 22.82 16.73 4.18
10-1004-90 100 µmol .086grams 1 20 12 7.5 5.45 4 1
10-1004-95 50 µmol .043grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1004-02 0.25 g .25grams 4.35 0.07 80.6 50.38 36.64 5.04
10-1004-90 100 µmol .086grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1004-95 50 µmol .043grams 0.75 0.07 8.6 5.38 3.91 0.54