3'-Acridine CPG

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20-2973
1-Dimethoxytrityloxy-2-(N-acridinyl-4-aminobutyl)-propyl-3-O-succinoyl-long chain alkylamino-CPG

Product Specifications

F.W.:
450.86
Catalog Number: Select Pack Size and Format to Reveal

Description

Acridine phosphoramidite is designed to produce an oligonucleotide containing acridine at any position in the molecule. Acridine CPG is used to label the 3'-terminus. Acridine is an effective intercalating agent.

Details

Usage

  • Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |(Acetyl-protected Cytidine should be used to avoid deamination when methanolic sodium hydroxide is used for deprotection.)
  • Deprotection: Cleavage and deprotection should be carried out using a mild deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support, neutralize with 1.5 volumes of 2M TEAA and desalt.|Note: NaOH is not compatible with dmf protecting groups.
Specifications
Storage Freezer storage, -10 to -30°C, dry



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