Dibenzo-cyclooctyne (DBCO) enables copper-free click chemistry, and our 3'-DBCO-Serinol CPG allows customers to place a DBCO modification at the 3'-end of their oligo without the use of a universal support. We recommend using 0.5M CSO oxidizer to prevent loss of DBCO label when using standard iodine oxidation.
Details
Usage
Coupling: DBCO Serinol is susceptible to damage by iodine during oxidation. To prevent loss of the DBCO label, use 0.5M CSO in anhydrous acetonitrile (40-4632-xx) and a 3 minute oxidation time for best results.
Deprotection: Ammonium hydroxide for 2 hr at 65°C or as required by nucleobases.