2'-OMe-U-CE Phosphoramidite

default
10-3130
5'-Dimethoxytrityl-Uridine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C40H49N4O9P
M.W.:
760.82
F.W.:
320.20
CAS Number:
110764-79-9
Catalog Number: Select Pack Size and Format to Reveal

Description

Glen Research 2'-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Details

Usage

  • Coupling: 6 minute coupling time recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 3-5 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3130-02 0.25 g .25grams 3.29 96.33 57.8 36.13 26.27 19.27 4.82
10-3130-05 0.5 g .5grams 6.57 205.67 123.4 77.13 56.09 41.13 10.28
10-3130-10 1.0 g 1grams 13.14 424.67 254.8 159.25 115.82 84.93 21.23
10-3130-90 100 µmol .076grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3130-02 0.25 g .25grams 4.9 0.07 91.6 57.25 41.64 5.73
10-3130-05 0.5 g .5grams 9.81 0.07 189.8 118.63 86.27 11.86
10-3130-10 1.0 g 1grams 19.62 0.07 386 241.25 175.45 24.13
10-3130-90 100 µmol .076grams 1.5 0.07 23.6 14.75 10.73 1.48