2’-OMe-G-Thiophosphoramidite

5'-Dimethoxytrityl-N-isobutyryl-Guanosine, 2'-O-methyl,3'-[(β-thiobenzoylethyl)-(1-pyrrolidinyl)]-thiophosphoramidite

Product Specifications

Formula:
C49H55N6O9PS2
M.W.:
967.10
F.W.:
391.36
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

The phosphorodithioate linkage (PS2) is both achiral and essentially resistant to nucleases. Previous studies have shown very interesting results which include observations that DNA with PS2 linkages activates RNase H , strongly inhibits human immunodeficiency virus (HIV) reverse transcriptase, induces B-cell proliferation and differentiation, and is completely resistant to hydrolysis by various nucleases. 2’-OMe- RNA Thiophosphoramidites are RNA monomers designed to produce oligos combining the PS2 linkage with the 2’-O-methyl ribose modification. These PS2-modified RNA oligos have potential for use in siRNAs and dithiophosphate aptamers (thioaptamers).

Details

Usage

  • Coupling: See Technical Bulletin
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_Thioamidites2015.pdf).
Specifications
Diluent 10% DCM in Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability Use Immediately


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3172-02 0.25 g .25grams 1.72 44 26.4 16.5 12 8.8 2.2
10-3172-90 100 µmol .097grams 0.67 9 5.4 3.38 2.45 1.8 0.45

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3172-02 0.25 g .25grams 2.57 0.1 45 28.13 20.45 2.81
10-3172-90 100 µmol .097grams 1 0.1 13.6 8.5 6.18 0.85