2'-OMe-A-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-Adenosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C48H54N7O8P
M.W.:
887.97
F.W.:
343.24
CAS Number:
110782-31-5
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research 2'-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Details

Usage

  • Coupling: 6 minute coupling time
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 3-5 DAYS


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3100-02 0.25 g .25grams 2.82 80.67 48.4 30.25 22 16.13 4.03
10-3100-05 0.5 g .5grams 5.63 174.33 104.6 65.38 47.55 34.87 8.72
10-3100-10 1.0 g 1grams 11.26 362 217.2 135.75 98.73 72.4 18.1
10-3100-90 100 µmol .089grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3100-02 0.25 g .25grams 4.2 0.07 77.6 48.5 35.27 4.85
10-3100-05 0.5 g .5grams 8.4 0.07 161.6 101 73.45 10.1
10-3100-10 1.0 g 1grams 16.81 0.07 329.8 206.13 149.91 20.61
10-3100-90 100 µmol .089grams 1.5 0.07 23.6 14.75 10.73 1.48