2'-OMe-5-F-U-CE Phosphoramidite

5'-Dimethoxytrityl-5-fluoro-Uridine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C40H48N4O9PF
M.W.:
778.78
F.W.:
338.19
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

To aid in the evaluation of the structures of 2'-OMe-RNA complexes, we offer several CE phosphoramidites. 2'-OMe-T is useful in triplex studies while the 2-aminopurine derivative may be tested in ribozyme studies. By supporting an additional hydrogen bond, 2,6-diaminopurine (2-amino-adenosine) binds more strongly with uridine than does adenosine. Oligonucleotides containing 2'-OMe-5-Me-C and 2'-OMe-I would be of interest to researchers involved in triplex and antisense studies using 2'-OMe-RNA. The uses of 2'-OMe-5-bromo-U phosphoramidite range from crystallographic studies due to the heavy atom to cross-linking because of its photolability. 5-Fluoro-pyrimidine nucleosides have been useful as therapeutic agents and their effect on the structure and activity of oligonucleotides may be examined using the 2'-OMe-RNA derivatives. The 2,4,6-trimethylphenyl (TMP) protected 2'-OMe-U derivative is a convertible nucleoside and reaction with ammonia leads to the 5-fluoro-dC analogue. 2'-OMe-3-deaza-5-aza-C (Reverse C) derivative has the potential to mimic in oligonucleotides 5-azacytidine, a DNA methylase inhibitor. Its ability to bind as a C will likely be diminished.

Details

Usage

  • Coupling: 6 minute coupling time
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent 20% DMF in Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 3-5 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3132-02 0.25 g .25grams 3.21 93.67 56.2 35.13 25.55 18.73 4.68
10-3132-90 100 µmol .078grams 1 20 12 7.5 5.45 4 1
10-3132-95 50 µmol .039grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3132-02 0.25 g .25grams 4.79 0.07 89.4 55.88 40.64 5.59
10-3132-90 100 µmol .078grams 1.5 0.07 23.6 14.75 10.73 1.48
10-3132-95 50 µmol .039grams 0.75 0.07 8.6 5.38 3.91 0.54