2,6-Diaminopurine-TOM-CE Phosphoramidite

5'-Dimethoxytrityl-N6-isobutyryl-N2-methoxyacetyl-2,6-diaminopurine riboside, 2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C57H81N8O11PSi
M.W.:
1113.36
F.W.:
344.22
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. The protecting scheme for 2,6-Diaminopurine has been changed and the original product (10-3084) has been replaced with the optimized product (10-3085) below.

Details

Specifications
Diluent Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3085-02 0.25 g .25grams 2.25 61.67 37 23.13 16.82 12.33 3.08
10-3085-90 100 µmol .111grams 1 20 12 7.5 5.45 4 1
10-3085-95 50 µmol .056grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3085-02 0.25 g .25grams 3.35 0.07 60.6 37.8 12.63 3.7
10-3085-90 100 µmol .111grams 1.5 0.07 23.6 14.7 4.92 1.4
10-3085-95 50 µmol .056grams 0.75 0.07 8.6 5.3 1.79 0.5