2',3'-ddA-CE Phosphoramidite

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10-7001
N6-diisobutylaminomethylidene-2',3'-dideoxyAdenosine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C28H47N8O3P
M.W.:
574.70
F.W.:
297.21
Catalog Number: Select Pack Size and Format to Reveal

Description

To avoid polymerase extension at the 3' terminus, 2',3'-dideoxynucleoside and 3'-deoxynucleoside CPGs have proved to be effective. 2',3'- Phosphoramidites are designed to be used with the 5'-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3'-OH) groups are removed. (3'-Termination can also be effected using a 3'-3' linkage formed using 5'-supports, or 3'-spacer C3 CPG.)

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: 17 hours in Ammonium Hydroxide at 55°C or 1 hour at 65°C in Ammonium Hydroxide/40% Methylamine 1:1 (v/v) (AMA).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-7001-02 0.25 g .25grams 4.35 131.67 79 49.38 35.91 26.33 6.58
10-7001-90 100 µmol .057grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-7001-02 0.25 g .25grams 6.49 0.07 123.4 77.13 56.09 7.71
10-7001-90 100 µmol .057grams 1.5 0.07 23.6 14.75 10.73 1.48