Trimer Phosphoramidite Mix 2

Mix of 19 Trimers (does not include TGC (Cys)) AAA/AAC/ACT/ATC/ATG/CAG/CAT/CCG/CGT/CTG/GAA/GAC/GCT/GGT/GTT/TAC/TCT/TGG/TTC (5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3:5.3)
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  • Coupling: 15 minute coupling time recommended.
  • Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.
Diluent Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.