Trimer Phosphoramidite Mix 1

Mix of 20 Trimers(AAA/AAC/ACT/ATC/ATG/CAG/CAT/CCG/CGT/CTG/GAA/GAC/GCT/GGT/GTT/TAC/TCT/TGC/TGG/TTC)(5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0)
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Description

All of the trimers are available individually so the researchers can prepare custom trimer mixes. Two pre-made catalog trimer mixes are available: 13-1991-xx, for incorporating all 20 amino acid codons equally into a sequence and 13-1992-xx, for incorporating 19 amino acid codons (-Cys). For a custom trimer mix of a particular subset of codons or a trimer mix that represents a set of trimers that is biased toward a particular codon or codons, please contact [email protected] for a quotation and projected delivery date.

Details

Usage

  • Coupling: 15 minute coupling time recommended.
  • Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.
Specifications
Diluent Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
13-1991-90 100 µmol 1 20 12 7.5 5.45 4 1
13-1991-95 50 µmol 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
13-1991-90 100 µmol 1.5 0.07 23.6 14.75 10.73 1.48
13-1991-95 50 µmol 0.75 0.7 8.6 5.38 3.91 0.54