2'-F-I-CE Phosphoramidite

5'-Dimethoxytrityl-deoxyInosine, 2'-fluoro-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C40H46FN6O7P
M.W.:
772.82
F.W.:
332.18
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

The significance of inosine in oligonucleotide synthesis comes from its ability to base pair with each of the standard bases (A, C, G, and T/U). Due to these unique pairing properties, inosine is considered as a “Universal Base” and has been an attractive candidate for many applications involving either synthetic primers or probes. This 2’-F version of inosine can be used in the same way as standard 2’-F phosphoramidites.

Details

Usage

  • Coupling: 3 minutes coupling time recommended.
  • Deprotection: Ammonium Hydroxide for 17 hours at 55°C or 30% Ammonia Hydroxide/40% Methylamine 1:1 (AMA) for 2 hours at Room Temperature. (Note - heating in AMA will lead to some degradation of the 2'-Fluoro nucleotides.)
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 Days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3440-90 100 µmol 077grams 1 20 12 7.5 5.45 4 1
10-3440-02 0.25 g .25grams 1.5 94.33 56.6 35.38 25.73 18.87 4.72

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3440-90E 100 µmol 077grams 3.23 0.07 23.6 14.75 10.73 1.48
10-3440-02E 0.25 g .25grams 4.83 0.07 90.2 56.38 41 5.64