5-Me-U-2'-MOE-Phosphoramidite

5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-uridine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C43H55N4O10P
M.W.:
818.90
F.W.:
378.27
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.

Details

Usage

  • Coupling: 6 minute coupling time recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 1-2 Days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3231-05 0.5 g .5grams 6.11 190.33 114.2 71.38 51.91 38.07 9.52
10-3231-10 1.0 g 1grams 12.21 393.67 236.2 147.63 107.36 78.73 19.68
10-3231-20 2.0 g 2grams 24.42 800.67 480.4 300.25 218.36 160.13 40.03

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3231-05E 0.5 g .5grams 9.11 0.07 175.8 109.88 79.91 10.99
10-3231-10E 1.0 g 1grams 0 0.07 358.2 223.88 162.82 22.39
10-3131-20E 2.0 g 2grams 0 0.07 722.6 451.63 328.45 45.16