G-2'-MOE-Phosphoramidite

5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-N2-isobutyryl- guanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C47H60N7O10P
M.W.:
914.01
F.W.:
403.29
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.

Details

Usage

  • Coupling: 6 minute coupling time recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3220-05 0.5 g .5grams 5.47 169 1.4 63.38 46.09 33.8 8.45
10-3220-10 1.0 g 1grams 10.94 351.33 210.8 131.75 95.82 70.27 17.57
10-3220-20 2.0 g 2grams 21.88 716 429.6 268.5 195.27 143.2 35.8

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3220-05E 0.5 g .5grams 8.16 0.07 156.8 98 71.27 9.8
10-3220-10E 1.0 g 1grams 16.33 0.07 320.2 200.13 145.55 20.01
10-3220-20E 1 g 2grams 32.66 0.07 646.8 404.25 294 40.43