5-Me-C-2'-MOE-Phosphoramidite

5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-N4-benzoyl- cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C50H60N5O10P
M.W.:
922.03
F.W.:
377.29
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.

Details

Usage

  • Coupling: 6 minute coupling time recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Note: Deprotection in AMA (50:50 Ammonium hydroxide/MethylAmine) may result in a 4-5% 5-methyl-N4-methyl-C side product at the modification site.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3211-05 0.5 g 0.5grams 5.42 167.33 100.4 62.75 45.64 33.47 8.37
10-3211-10 1.0 g 1grams 10.85 348.33 209 130.63 95 69.67 17.42
10-3211-20 2.0 g 2grams 21.69 709.67 425.8 266.13 193.55 141.93 35.48

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3211-05E 0.5 g 0.5grams 8.09 0.07 155.4 97.13 70.64 9.71
10-3211-10E 1.0 g 1grams 16.19 0.07 317.4 198.38 144.27 19.84
10-3211-20E 2.0 g 2grams 32.38 0.07 641.2 400.75 291.45 40.08