A-2'-MOE-Phosphoramidite

5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-N6-benzoyl-adenosine -3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C50H58N7O9P
M.W.:
932.03
F.W.:
387.29
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.

Details

Usage

  • Coupling: 6 minute coupling time recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3200-05 0.5 g 0.5grams 5.3 165.33 99.2 62 45.09 33.07 8.27
10-3200-10 1.0 g 1grams 10.73 344.33 206.6 129.13 93.91 68.87 17.22
10-3200-20 2.0 g 2grams 21.46 702 421.2 263.25 263.25 140.4 35.1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3200-05E 0.5 g 0.5grams 8.01 0.07 153.8 96.13 69.91 9.61
10-3200-10E 1.0 g 1grams 16.01 0.07 313.8 196.13 142.64 19.61
10-3200-20E 2.0 g 2grams 32.03 0.07 634.2 396.38 288.27 39.64