2'-OMe-ibu-G-CE Phosphoramidite

5'-Dimethoxytrityl-N-isobutyryl-Guanosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
main product photo

Product Specifications

Formula:
C45H56N7O9P
M.W.:
869.97
F.W.:
359.24
CAS Number:
150780-67-9
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Safety Data Sheet

Glen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNA

Glen Report 5-11: 2'-0Me-RNA -The Logical RNA Alternative?

Glen Report 4-13: RNA Synthesis - 2'-O-Methyl Analogues

Glen Report 4-14: Literature Review

Glen Report 35-16: Technical Note — Sugar Conformations and Modifications

Description

Glen Research 2'-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Details

Usage

  • Coupling: 6 minute coupling time
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 3-5 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3120-02 0.25 g .25grams 2.87 82.33 49.4 30.88 22.45 16.47 4.12
10-3120-05 0.5 g .5grams 5.75 178.33 107 66.88 48.64 35.67 8.92
10-3120-10 1.0 g 1grams 11.49 369.67 221.8 138.63 100.82 73.93 18.48
10-3120-90 100 µmol .087grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3120-02 0.25 g .25grams 4.29 0.07 79.4 49.63 36.09 4.96
10-3120-05 0.5 g .5grams 8.58 0.07 165.2 103.25 75.09 10.33
10-3120-10 1.0 g 1grams 17.16 0.07 336.8 210.5 153.09 21.05
10-3120-90 100 µmol .087grams 1.5 0.07 23.6 14.75 10.73 1.48

References

tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells Musa M. Mhlanga1, Diana Y. Vargas, Cindy W. Fung, Fred Russell Kramer and Sanjay Tyagi

Easily denaturing nucleic acids derived from intercalating nucleic acids: thermal stability studies, dual duplex invasion and inhibition of transcription start Vyacheslav V. Filichev, Birte Vester, Lykke H. Hansen, and Erik B. Pedersen

IPyrene binary probes for unambiguous detection of mRNA using time-resolvedû fluorescence spectroscop Angel A. Marti, Xiaoxu Li, Steffen Jockusch, Zengmin Li, BinduûRaveendra, Sergey Kalachikov, James J. Russo, Irina Morozova, Sathyanarayanan V. Puthanveettil, Jingyue Ju, and Nicholas J. Turro

The chemical stability of abasic RNA compared to abasic DNA Pascal A. Kupfer and Christian J. Leumann

Three essential and conserved regions of the group II intron are proximal to the 5'-splice site Alexandre de Lencastre and Anna Marie Pyle

Novel Photodynamic Effect of a Psoralen-Conjugated Oligonucleotide for the Discrimination of the Methylation of Cytosine in DNA Asako Yamayosh, Yohei Matsuyama, Mikihiko Kushida, Akio Kobori and Akira Murakami

Kinetic and subcellular analysis of PS-ASO/protein interactions with P54nrb and RNase H1 Timothy A Vickers, Meghdad Rahdar, Thazha P Prakash, Stanley T Crooke,

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