Glen Research offers a range of different 5’-Amino-Modifiers designed for use in automated synthesizers to functionalize the 5’-terminus of a target oligonucleotide with a primary amine. These modifiers vary based on the amine protecting group and the linker. Of these, the trifluoroacetyl (TFA)-protected Amino-Modifiers are typically used when a trityl group is not necessary. The TFA group allows deprotection to be completed in a single standard step to give an amine that is immediately available for conjugation with amine-reactive agents.
The 5’-Amino-Modifier TEG has a 12-atom hydrophilic triethylene glycol linker with a TFA protecting group.
It should be noted that TFA-amino modifiers are sensitive to prolonged transit times and elevated temperatures (e.g., international shipments in the summer). Under these conditions, PDA-amino modifiers are significantly more stable and are excellent alternatives (https://www.glenresearch.com/reports/gr25-16).
|Storage||Freezer storage, -10 to -30°C, dry|
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
|Catalog #||Pack Size||Grams/Pack||0.1M Dil. (mL)||Approximate Number of Additions|
|Catalog #||Pack Size||Grams/Pack||Dilution (mL)||Approximate Number of Additions|