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CleanAmp™-Ac-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-2'-deoxyCytidine,3'-[(4-oxo-tetradecyl)-(N,N-diisopropyl)]-phosphoramidite

This product has been discontinued.

We have discontinued this product permanently. Please find recommended products below.
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Product Specifications

Formula:
C52H73N4O9P
M.W.:
929.13
F.W.:
FW: 499.54 (triester)
To Retrieve a Catalog Number, Select a Pack Size and Format:

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Technical Documents

Safety Data Sheet

Glen Report 21.11: Hot Start PCR update: CleanAmp™ Primers

Glen Report 21.12: Procedure for Synthesis of CleanAmp™ Primers

Glen Report 21.22: Purification of CleanAmp™ DNA Oligonucleotides (DMT-ON)

Description

CleanAmp™ Primers offer an alternative to other Hot Start technologies and allow greater control of primer hybridization and extension during PCR. It has been demonstrated that CleanAmp™ Primers outperform other technologies in multiple applications. Indeed, over a broad range of applications, CleanAmp™ Primers reduce or eliminate off-target amplification. Greater amplicon yield is also achieved, due to improvement in specificity and sensitivity. By using either the slow-releasing Precision primers with two CleanAmp™ phosphotriester linkages or the faster-releasing Turbo Primers with a single CleanAmp™ phosphotriester linkage, the rate of formation of unmodified primer can be controlled to suit reaction needs. A table to aid in the selection of Turbo and Precision Primers for specific applications is shown below.

Turbo Primers Precision Primers
Fast cycling Standard cycling
Multiplex PCR One-step reverse-transcription PCR
Improves amplicon yield Improved specificity and limit of detection
Reduces mis-priming/ primer dimer formation Greatest reduction in mis-priming/primer dimer formatio

Synthesis of CleanAmp™ Primers requires the use of UltraMild Chemistry.

Details

Usage

  • Coupling: 10 minute coupling time. CleanAmp™ monomers must be used with UltraMild phosphoramidites and Cap Mix.
  • Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Glen Report 21.2 for details. (http://www.glenresearch.com/GlenReports/GR21-2.pdf)
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours

Intellectual Property

CleanAmp™ is a trademark of TriLink BioTechnologies, Inc. CleanAmp™ products or portions thereof are covered by TriLink pending Patent Applications, US 2007281308 and WO2007139723, US Provisional Patent Application Serial # 61/056,324 and US Patent 6762298 licensed from the Department of Health and Human Services. CleanAmp™ products are sold exclusively for R & D use by the purchaser. They may not be resold, distributed or re-packaged. No license is granted or implied with the purchase of this product. Amplification methods used in connection with Polymerase Chain Reaction (“PCR”) Process are covered by many patents. It may be necessary to obtain a separate license for certain patented applications in which the product is used. CleanAmp™ Licenses are available directly from TriLink BioTechnologies.

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1450-02 0.25 g .25grams 2.69 76.33 45.8 28.63 20.82 15.27 3.82
10-1450-05 0.5 g .5grams 5.38 166 99.6 62.25 45.27 33.2 8.3
10-1450-90 100 µmol .093grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1450-02 0.25 g .25grams 4.02 0.07 74 46.25 33.64 4.63
10-1450-05 0.5 g .5grams 8.03 0.07 154.2 96.38 70.09 9.64
10-1450-90 100 µmol .093grams 1.5 0.07 23.6 14.75 10.73 1.48