CleanAmp™-Pac-dA-CE Phosphoramidite

CleanAmp™ Primers offer an alternative to other Hot Start technologies and allow greater control of primer hybridization and extension during PCR. It has been demonstrated that CleanAmp™ Primers outperform other technologies in multiple applications. Indeed, over a broad range of applications, CleanAmp™ Primers reduce or eliminate off-target amplification. Greater amplicon yield is also achieved, due to improvement in specificity and sensitivity. By using either the slow-releasing Precision primers with two CleanAmp™ phosphotriester linkages or the faster-releasing Turbo Primers with a single CleanAmp™ phosphotriester linkage, the rate of formation of unmodified primer can be controlled to suit reaction needs. A table to aid in the selection of Turbo and Precision Primers for specific applications is shown below. Turbo Primers Precision Primers Fast cycling Standard cycling Multiplex PCR One-step reverse-transcription PCR Improves amplicon yield Improved specificity and limit of detection Reduces mis-priming/ primer dimer formation Greatest reduction in mis-priming/primer dimer formation Synthesis of CleanAmp™ Primers requires the use of UltraMild Chemistry.