dC-H-Phosphonate, DBU Salt

5'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine,3'-H-phosphonate, DBU salt

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Product Specifications

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Glen Research H-Phosphonates are analyzed by HPLC and are synthesis-tested. H-Phosphonates are especially useful for the preparation of modified internucleotide linkages which are unattainable by phosphoramidite chemistry. The most popular application is the preparation of radiolabelled phosphorothioates, since the sulfurization reaction is carried out off the synthesizer.



  • Coupling: H-Phosphonate Synthesis Protocol.|(The DBU is now being provided in place of the TEA salt for dC-H Phosphonate. This product shows better monomer stability and solubility.)
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Diluent Acetonitrile/Pyridine (1:1)
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability ~1week

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1210-02 0.25 g .25grams 21 686.67 412 257.5 187.27 137.33 34.33
10-1210-05 0.5 g .5grams 42 1386.67 832 520 378.18 277.33 69.33


Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1210-02 0.25 g .25grams 5 0.06 93.6 58.5 42.55 5.85
10-1210-05 0.5 g .5grams 10 0.06 193.6 121 88 12.1

Product FAQs

  • What are the concentrations of the solutions used on an ABI for H-phosphonate chemistry?