5'-Dimethoxytrityloxy-5-[N-((tetramethylrhodaminyl)-aminohexyl)-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Rhodamine derivatives are not sufficiently stable to survive conventional deprotection and these must be attached to amino-modified oligonucleotides using post-synthesis labelling techniques. Because Tetramethyl Rhodamine (TAMRA) is not base stable, the procedure to cleave and deprotect the labelled oligonucleotide must be carefully considered. Using the UltraMILD monomers and deprotection with potassium carbonate in methanol, TAMRA oligonucleotides can be fairly conveniently isolated. To streamline the preparation of TAMRA oligos, we offer 3'-TAMRA CPG for 3' labelling and TAMRA-dT for labelling within the sequence. We also offer TAMRA NHS ester for labelling amino-modified oligonucleotides.
The spectral characteristics of this dye is detailed here.
| Specifications | |
|---|---|
| Diluent | 10% THF in Anhydrous Acetonitrile |
| Storage | Freezer storage, -10 to -30°C, dry |
| Stability | 24 hours |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1057-02 | 0.25 g | .25grams | 1.91 | 50.33 | 30.2 | 18.88 | 13.73 | 10.07 | 2.52 |
| 10-1057-90 | 100 µmol | .131grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1057-95 | 50 µmol | .066grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1057-02 | 0.25 g | .25grams | 2.85 | 0.07 | 50.6 | 31.63 | 23 | 3.16 |
| 10-1057-90 | 100 µmol | .131grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
| 10-1057-95 | 50 µmol | .066grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
