5'-Dimethoxytrityl-N4-(O-levulinyl-6-oxyhexyl)-5-Methyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
A branching monomer is required to construct comb-like oligonucleotide probes. The developers of the comb system from Chiron Corporation evaluated1 several protecting groups for the branch point and chose levulinyl (LEV), which is specifically removed using a reagent containing hydrazine hydrate, acetic acid and pyridine.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability | 24 hours |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1018-02 | 0.25 g | .25grams | 2.65 | 75 | 45 | 28.13 | 20.45 | 15 | 3.75 |
| 10-1018-90 | 100 µmol | .094grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1018-02 | 0.25 g | .25grams | 3.96 | 0.07 | 72.8 | 45.5 | 33.09 | 4.55 |
| 10-1018-90 | 100 µmol | .094grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
REFERENCE(S)| 1 T. Horn, C.A. Chang, and M.S. Urdea, Nucleic Acids Res, 1997, 25, 4842-4849.
