1-Methyl-PseudoUridine Phosphoramidite

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10-3056
5'-Dimethoxytrityl-1-methyl-pseudoUridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C46H63N4O9PSi
M.W.:
875.07
F.W.:
320.19
CAS Number:
875302-45-7
Catalog Number: Select Pack Size and Format to Reveal

Description

RNA methylation occurs in a large selection of RNA nucleosides and this post transcriptional modification of RNA, carried out by a variety of RNA methyltransferases, appears in a wide variety of RNA species - including tRNA, mRNA, miRNA and RNA viruses. Over 90 methylated nucleosides have been found in tRNA and these play many significant roles in tRNA structure. In addition, methylation appears to mark the tRNA as mature, preventing its degradation as well as directing localization within the cell. mRNA, modified with 1-methylpseudouridine (1-Me-Ψ ) alone or in combination with 5-methylcytidine (5-Me-C), significantly increases protein expression in cells and mouse models. 1-Me-Ψ is also a modified nucleobase that can greatly enhance the properties of mRNA by reducing immunogenicity and increasing stability.

Details

Usage

  • Coupling: 12 minute coupling time recommended.
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3056-02 0.25 g .25grams 2.86 82 49.2 30.75 22.36 16.4 4.1
10-3056-90 100 µmol .088grams 1 20 12 7.5 5.45 4 1
10-3056-95 50 µmol .044grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3056-02 0.25 g .25grams 4.26 0.07 78.8 49.2 16.42 4.9
10-3056-90 100 µmol .088grams 1.5 0.07 23.6 14.7 4.92 1.4
10-3056-95 50 µmol .044grams 0.75 0.07 8.6 5.3 1.79 0.5