1-Me-dA-CE Phosphoramidite

5'-Monomethoxytrityl-N6-chloroacetyl-1-methyl-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C42H49ClN7O6P
M.W.:
814.31
F.W.:
328.24
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Cellular polynucleotides are alkylated by endogenous components, such as S-adenosylmethionine, or after reacting with two general classes of environmental and laboratory chemicals. SN1 chemical agents include alkylnitrosourea and N-alkyl-N-nitro-N-nitrosoguanidine that react with the N7 position of guanine, N3 of adenine, O6 of guanine, O2 or O4 of pyrimidines, and the non-phosphodiester oxygen atoms of the phosphate backbone. In contrast, SN2 chemical agents such as methyl methanesulfonate and dimethyl sulfate react primarily with the N1 position of adenine (1-Methyl-2'-deoxyadenosine) and N3 of cytosine. To avoid chain branching during synthesis when using DCI as activator, N6-Me-dA is offered with acetyl protection.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |Monomers that allow for UltraMILD deprotection are recommended. (dA:10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). 0.02M Iodine for oxidation is also recommended.
  • Deprotection: 2 M Ammonia in methanol, 24 hours at Room Temperature using UltraMILD amidites or 60 hours at Room Temperature if using standard amidites.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability Similar to dA,C,G,T-CE Phosphoramidites


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1501-02 0.25 g .25grams 3.07 89 53.4 33.38 24.27 17.8 4.45
10-1501-90 100 µmol .081grams 1 20 12 7.5 5.45 4 1
10-1501-95 50 µmol .041grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1501-02 0.25 g .25grams 4.58 0.07 85.2 53.25 38.73 5.33
10-1501-90 100 µmol .081grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1501-95 50 µmol .041grams 0.75 0.07 8.6 5.38 3.91 0.54