Phosphorodithioate (PS2-oligos) require the use of thiophosphoramidite monomers during synthesis. The thiophosphite linkage formed on coupling of a thiophosphoramidite is sulfurized to the dithioate prior to the next coupling cycle. The chemistry is challenging but the reward is the formation of an achiral linkage that has demonstrated many interesting properties that may be most relevant to the synthesis of aptamers. The monomers are now available.

'Click Chemistry' refers to the [3+2] cycloaddition between alkynes and azides, a reaction which has allowed remarkable selectivity in conjugation reactions in biological samples. Glen Research already offers a simple hexynyl phosphoramidite for adding an alkyne group to oligonucleotides. Azides are not compatible with phosphoramidite additions and so we are now adding two products to overcome this: (1) bromohexyl phosphoramidite as an intermediate to prepare a 5'-azido-modified oligonucleotide; and (2) azidobutyrate NHS ester to add an azido group to any amino-modified oligonucleotide by post synthesis conjugation.

8-D-dG-CE Phosphoramidite	Formylindole
Solid Chemical Phosphorylation Reagent
5'-CholesterylTEG Phosphoramidite

Technical Briefs

Glen-Pak purification of 6-FAM oligos

New application for 5-Me-iso-dC and iso-dG

New application for 5'-OMe-dT

Expansion of the genetic alphabet by unnatural base pair systems offers great potential for the development of duplex DNA and proteins with unique properties. Nucleic acids and proteins with extra components at specific positions could exhibit novel or increased functionality, which would be useful for both basic research and applied sciences. This is part of the new 'Synthetic Biology'. Glen Research is already a source for nucleoside derivatives related to unnatural base pairs, such as dP, dK, 5-Me-iso-dC, and iso-dG. In association with TagCyx Biotechnologies, we are adding to our lineup the amidites of dDs, dPa, and ds, the elements for a new unnatural base pair system based on complementarity using specific hydrophobicity and shape.

RECENT HIGHLIGHTS

A Comparative Study of Universal Supports

Pyrrolidine CE Phosphoramidite

Pyrene-dU, Perylene-dU

Fmoc-Amino-Modifier C6-dT

Large Scale Synthesis

Long Trebler

New Amino-Modifier

NHS-Carboxy-dT

AP-dC (G-clamp) UPDATE

Oligonucleotide Caps

Sulfurizing Reagent II

dC-BRANCHER

High-Efficiency Purification

Black Hole Quenchers™

2'-Fluoro RNA

PREVIOUS Technical Briefs

RNA

Activators

Four-color DNA sequencing with 3'-O-modified nucleotide reversible , terminators and chemically cleavable fluorescent dideoxynucleotides

Jia Guo, Ning Xu, Zengmin Li, Shenglong Zhang, Jian Wu, Dae Hyun Kim, Mong Sano Marma, Qinglin Meng, Huanyan Cao, Xiaoxu Li, Shundi Shi, Lin , Yu, Sergey Kalachikov, James J. Russo, Nicholas J. Turro, and Jingyue , Ju

PNAS. 2008; 105(27): p. 9145-9150 Open Access
Supplement

conferences and posters