Glen Research Product Information

Catalog Number: 50-5910-xx
Description: TAMRA NHS Ester

Carboxytetramethylrhodamine, N-hydroxysuccinimide ester in dimethyl sulfoxide (DMSO)

Formula: C29H25N3O7 in DMSO 
M.W.: 527.53 
F.W.: 413.45 

Diluent: ~0.17M Solution in Anhydrous DMSO

Coupling: Conjugate with an amino-modified oligo in sodium carbonate/bicarbonate buffer (pH=9). See Technical Bulletin for details.

Deprotection: not applicable

Storage: Freezer storage, -10 to -30°C, dry


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QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see

Oligonucleotide Properties Calculator;

QUESTION: What are the relative extinction coefficients of various dyes?

RESPONSE:Please see

QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?

RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%

QUESTION: Dye Quencher Plot

RESPONSE:Dye Quencher Plot






















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