Glen Research Product Information

Catalog Number: 50-1960-xx
Description: Methylene Blue NHS Ester

3-(N,N-dimethylamino)-7-[N-(3-(N-succinimidyl)-carboxyethyl)- N-(methyl)-amino]-phenothiazin-5-ium perchlorate

Formula: C22H23N4O8SCl 
M.W.: 538.96 
F.W.: 425.89 

Diluent: Acetonitrile, DMSO, DMF

Labeling: For labeling 0.2µmoles of an amino-modified oligonucleotide, dissolve 5.4mg of the Methylene Blue NHS Ester in 60µL of DMSO (Acetonitrile or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500µL 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12µL of the Methylene Blue NHS Ester solution and vortex. Let react for 2 hours at room temperature and desalt.

Storage: Freezer storage, -10 to -30°C, dry

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


50-1960-23
5.4mg
540.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html


 

 

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01/18/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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