Diluent: 60% pyridine/40% Acetonitrile (fully dissolve in pyridine first, see notes below*)
Sulfurizing: A 60 second sulfurization step time for DNA or a 360 second sulfurization step time for RNA is recommended. Sulfurization step must come before capping step to minimize phosphodiester formation.
Deprotection: See TB_Sulfurizing II.pdf
Storage: Controlled room temperature or lower, dry
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.