2-[2-(4,4'-Dimethoxytrityoxy)ethylsulfonyl]ethyl-2-succinoyl)- long chain alkylamino-CPG 500
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. Note: complete deprotection to the 3'-phosphate requires 17 hr @ RT or 4 hr @ 55°C in ammonium hydroxide, or 8 hr @ RT in K2CO3/MeOH