Coupling: UltraMILD monomers should be used. (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/40-4212-xx).
Deprotection: Deprotect with 30% Ammonium Hydroxide for 2 hours at Room Temperature. Note: BHQ-3 is unstable to potassium carbonate in methanol.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: 'Black Hole Quencher', 'BHQ-0', 'BHQ-1', 'BHQ-2' and 'BHQ-3' are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc.. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged.