Coupling: No changes needed from standard method recommended by synthesizer manufacturer. Use 0.02M Iodine to avoid degradation of the cyanine dye.
Deprotection: As required by nucleobases. If dmf-dG is used during synthesis, deprotect in Ammonium Hydroxide for 2 hours at 65 °C; with ibu-dG, deprotect for 24-36 hours at room temperature. Note Cyanine 3 is compatible with UltraFast deprotection (AMA 10 minutes at 65 °C) if Ac-dC was used.