Glen Research Product Information

Catalog Number: 20-5910-xx
Description: 3'-TAMRA CPG

1-Dimethoxytrityloxy-3-[O-(N-carboxy-(Tetramethyl-rhodamine)- 3-aminopropyl)]-propyl-2-O-succinoyl-long chain alkylamino-CPG

 
 
F.W.: 623.60 

Coupling: Use either dmf-dG (10-1029-xx) with standard monomers and Cap A or use UltraMILD monomers (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ) and UltraMILD Cap Mix A (40-4210-xx/ 40-4212-xx).

Deprotection: If standard monomers were used, deprotect in tert-butylamine/water 1:3 (v/v) for 6 hours at 60 °C.  If UltraMILD monomers were used, deprotect in 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_UltraMild_Deprotection.pdf). Note: A side reaction can occur that leads to a loss of the TAMRA label. Treatment of the support prior to cleavage/deprotection with a hindered base, such as 10% diethylamine in ACN, for 3-5 minutes will minimize the loss of label.

Storage: Freezer storage, -10 to -30°C, dry. Light sensitive material.

Stability in Solution: Not Applicable

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


20-5910-01
0.1g
120.00
20-5910-10
1.0g
995.00
20-5910-41
Pack of 4
200.00
20-5910-42
Pack of 4
120.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html


QUESTION: What are the relative extinction coefficients of various dyes?

RESPONSE:http://www.glenres.com/Technical/Extinctions.html#dyes


QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?

RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%


QUESTION: Dye Quencher Plot

RESPONSE:Dye Quencher Plot


 

 

VIEW PRINTABLE VERSION

 

 


  

01/18/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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