5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-acetate-3'-succinoyl- long chain alkylamino-CPG
Coupling: Please refer to the technical bulletin for the synthesis and deprotection procedures. This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.
QUESTION: Are any changes required from standard DNA synthesis and deprotection?
RESPONSE:These supports (20-3303, 20-3315, 20-3324, 20-3330) can be used for oligodeoxynucleotide synthesis when a ribose functionality is desired at the 3'-terminus. Following periodate oxidation of the 1,2-diol, labelling or carrier molecules containing amino groups can be conjugated to the 3'-terminus. An example (1) is the use of poly-lysine as a carrier molecule.
These supports can be used for RNA synthesis, and our other RNA supports (now all containing 2'-acetyl protecting groups, which are completely base-labile) can also be used for DNA modification.
(1a) M. Lemaitre, B. Bayard, and B. Lebleu, Proc. Natl. Acad. Sci. USA, 1987, 84, 648-652.
(1b) M. Lemaitre, C. Bisbal, B. Bayard, and B. Lebleu, Nucleosides & Nucleotides, 1987, 6, 311-315.