Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Use ammonium hydroxide and deprotect as required by nucleobases. When using AMA, a small amount of a non-fluorescent impurity will be formed. To eliminate this impurity, first deprotect with ammonium hydroxide for 30 minutes at room temperature, add an equal volume of 40% methylamine and then complete the deprotection as required by the nucleobases - e.g. 10 minutes at 65°C or 2 hours at room temperature for standard bases.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: Not Applicable
Please Note: This product is covered by US Patent No.: 8,394,948.