Glen Research Product Information

Catalog Number: 20-2963-xx
Description: 3'-Fluorescein CPG

1-Dimethoxytrityloxy-2-(N-thiourea-(di-O-pivaloyl-fluorescein)- 4-aminobutyl)-propyl-3-O-succinoyl-long chain alkylamino-CPG

 
 
F.W.: 598.56 

Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.

Deprotection: Use ammonium hydroxide and deprotect as required by nucleobases. When using AMA, a small amount of a non-fluorescent impurity will be formed. To eliminate this impurity, first deprotect with ammonium hydroxide for 30 minutes at room temperature, add an equal volume of 40% methylamine and then complete the deprotection as required by the nucleobases - e.g. 10 minutes at 65°C or 2 hours at room temperature for standard bases.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: Not Applicable

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


20-2963-01
0.1g
120.00
20-2963-10
1.0g
995.00
20-2963-41
Pack of 4
200.00
20-2963-42
Pack of 4
120.00
20-2963-13
Pack of 1
300.00
20-2963-14
Pack of 1
450.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html


QUESTION: What are the relative extinction coefficients of various dyes?

RESPONSE:Please see http://www.glenres.com/Technical/Extinctions.html#dyes


QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?

RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%


 

 

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01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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