Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Cleavage of the oligonucleotide from this support using ammonium hydroxide requires overnight at 55°C or 48 hours at room temperature; in AMA, 10 minutes at 65°C or 2 hours at room temperature is required. This treatment will complete the deprotection of standard nucleobases. For additional cleavage conditions, see Technical Bulletin (http://www.glenres.com/GlenReports/GR15-12.html).
Storage: Freezer Storage, -10 to -30°C, dry
Stability in Solution: Not Applicable