Glen Research Product Information

Catalog Number: 20-2900-xx
Description: 3'-Phosphate CPG

2-[2-(4,4'-Dimethoxytrityloxy)ethylsulfonyl]ethyl-2-succinoyl)- long chain alkylamino-CPG

 
 
F.W.: 79.98 

The 3'-Phosphate supports are not compatible with ß-elimination techniques such as 10% diethylamine in acetonitrile to remove cyanoethyl groups without significant yield loss. Use 3'-CPR II CPG (20-2903) instead. Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.

Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. Note: complete deprotection to the 3'-phosphate requires 17 hr @ RT or 4 hr @ 55°C in ammonium hydroxide, 30 minutes at 65 °C in AMA (Ammonium hydroxide/40% methylamine 1:1 v/v), or 8 hr @ RT in K2CO3/MeOH.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: Not Applicable

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


20-2900-01
0.1g
70.00
20-2900-10
1.0g
480.00
20-2900-41
Pack of 4
100.00
20-2900-42
Pack of 4
60.00
20-2900-13
Pack of 1
180.00
20-2900-14
Pack of 1
280.00

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09/24/2018

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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