2-[2-(4,4'-Dimethoxytrityloxy)ethylsulfonyl]ethyl-2-succinoyl)- long chain alkylamino-CPG
The 3'-Phosphate supports are not compatible with ß-elimination techniques such as 10% diethylamine in acetonitrile to remove cyanoethyl groups without significant yield loss. Use 3'-CPR II CPG (20-2903) instead.
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. Note: complete deprotection to the 3'-phosphate requires 17 hr @ RT or 4 hr @ 55°C in ammonium hydroxide, 30 minutes at 65 °C in AMA (Ammonium hydroxide/40% methylamine 1:1 v/v), or 8 hr @ RT in K2CO3/MeOH.